PCR testing laboratory design and installation
PCR testing laboratory design and installation
The PCR pharma machinery and the PCR reagent production area should be in separate buildings or spaces to ensure that the air is not directly connected to prevent cross-contamination caused by aerosols formed during amplification; the PCR testing pharma machinery should set up the following areas: reagents Storage and preparation areas, specimen preparation areas,...
Text label: PCR testing laboratory, PCR laboratory design and installation, PCR laboratory decoration company
PCR testing pharma machinery design and installation
The PCR laboratory and the PCR reagent production area should be in separate buildings or spaces to ensure that the air is not directly connected to prevent cross-contamination caused by aerosols formed during amplification; the PCR testing laboratory should set up the following areas: reagents Storage and preparation area, sample preparation area, amplification area, and amplification product analysis area, the areas can be appropriately combined according to the functions of the instruments used; each area should be completely independent of each other in physical space, whether each area is in space or in In use, it should always be in a completely separated state, it should not be just a formal partition, and it should not be one area nested within one area; each area should not be directly connected with air, and the amplification area and the amplification product analysis area should not be in each area. It is advisable to use the independent direct exhaust method to discharge the air. If the air-conditioning unit is used, the PCR laboratory should have an independent air-conditioning unit; at the same time, the possibility of air connection in each room after the shutdown should be considered, and necessary control measures should be taken.
Functions of the reagent storage and preparation area: preparation of storage reagents, subpackage of reagents and preparation of amplification reaction mixture, as well as storage and preparation of consumables such as centrifuge tubes and pipette tips. Ancillary supplies should generally include: refrigerators with a temperature control range of 2°C to 8°C and/or below -20°C; micro-sampler; mixer; UV disinfection equipment; special work clothes and work shoe covers; disposable gloves, durable Autoclaved centrifuge tubes and pipette tips; specialty office supplies. The functions of the amplification area: DNA synthesis, DNA amplification and detection; supporting supplies generally include: nucleic acid amplification instrument; micro-sampler; ultraviolet disinfection equipment; Sampler tip with filter element; special work clothes and work shoes; special office supplies. Zhongjing Global Purification can provide consulting, planning, design, construction, installation, transformation and other supporting services for PCR laboratories and PCR testing laboratories.
Functions of the specimen preparation area: nucleic acid RNA, DNA extraction, storage and sample addition; ancillary supplies generally include: temperature control range of 2°C to 8°C and refrigerators below -20°C; high-speed centrifuges; mixers; water baths Chamber or heating module; micropipette; UV disinfection equipment; biological safety cabinet; disposable gloves, autoclave centrifuge tubes and pipette tips with filter; special work clothes and work shoes; special office supplies; if necessary Macromolecular DNA should have a sonicator. Functions of amplified product analysis area: further analysis and determination of amplified fragments; depending on the test method, basic configuration: micro-sampler; UV disinfection equipment; disposable gloves, disposable caps, sampler tips with filter elements ; Special work clothes and work shoes; Special office supplies. Equipment maintenance procedures should be established, and measurement equipment should be regularly tested. For example, nucleic acid amplification instruments, micro-samplers, biological safety cabinets, and centrifuges should be tested or calibrated every year.
The cleaning of the laboratory should be carried out in the direction of reagent storage and preparation area→specimen preparation area→amplification area→amplification product analysis area. Different experimental areas should have their own cleaning tools to prevent cross-contamination; store reagents Materials such as consumables and consumables for specimen preparation should be directly transported to the reagent storage and preparation area, and cannot pass through the amplification detection area. Consumables such as sample addition tips and PCR reaction tubes should be handled to prevent contamination; The reference substance and quality control substance should be stored in the specimen processing area, the reagents should use molecular biology grade reagents, and the purified water used should be autoclaved; in order to avoid cross-contamination between samples, after adding the nucleic acid to be tested, the reagents containing the reaction should be covered. The reaction tube of the mixed solution; the pollution caused by aerosol should be avoided, the movement in the amplification area should be minimized, and the amplification reaction tube should not be opened in the amplification area.
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