Degradation and preservation of edible fungus factory strains

Degradation and preservation of edible fungus factory strains

In the process of passage of edible fungus factory strains, due to changes in genetic material, the original excellent characters gradually disappear or deteriorate, resulting in poor growth, late fruiting, low yield, poor quality, and clustered fruit bodies. Such phenomena are called degradation; the degradation of bacteria is often a gradual process...

Text label: Edible mushroom factory design, Edible mushroom factory decoration, Degradation preservation of bacteria

Degradation and preservation of edible fungus factory strains

In the process of passage of edible fungus factory strains, due to changes in genetic material, the original excellent characters gradually disappear or deteriorate, resulting in poor growth, late fruiting, low yield, poor quality, and clustered fruit bodies. Such phenomena are called degradation; bacterial species degradation is often a gradual process, and bacterial species degradation will only be revealed when individuals with harmful mutations increase significantly in the population and become dominant; the reason for bacterial species degradation: bacterial species Promiscuous, deleterious mutations, and hybrid strains have imbalanced ratios of nucleophiles. Measures to prevent bacterial species degradation: To prevent the mixing of bacterial species, during the transfer of bacterial species and the fruiting test, etc., the isolation of varieties should be strengthened to reduce the mixing between varieties, so as to ensure that good varieties can be maintained for a long time. Sufficient stability; control the number of bacterial species passages, the greater the number of bacterial species passages, the greater the probability of mutation, so the greater the probability of bacterial species degradation, the production should strictly control the transfer generation number of bacterial species; using effective The strain preservation method should be a combination of short-term, medium-term and long-term, and different preservation methods should be used for expansion and transfer according to different requirements to ensure that the strain can maintain the original variety for a long time. Excellent traits; create good strains to produce good nutritional conditions and external environment.

Maintaining the excellent characters and the characteristics of strong vitality is an important condition to ensure the high quality and high yield of edible fungi. In order to avoid the degradation of edible fungi, measures of rejuvenation must be taken: systematic breeding, and selection of typical varieties of this species in production The young fruiting bodies of the traits are separated, and new pure mycelia are recovered, and the original species are preserved as much as possible, and are properly preserved; the tip of the mycelium is separated, and the top part of the robust mycelium is picked, and the tube is purified and cultured. In order to maintain the purity of the strain, restore the original excellent species and viability of the strain to achieve the purpose of rejuvenation; change the culture formula, and constantly change the formula of the medium during the separation, preservation and subculture process of the strain. Bacteria that have been cultivated for several generations on the same medium for a long time can gradually decline in life. The carbon source, carbon-nitrogen ratio, microorganisms, and mineral nutrients are properly adjusted, and the bacteria that have declined due to the malnutrition mechanism can be rejuvenated to a certain extent. In the process of isolation, culture and preservation of strains, closely observe the growth status of mycelium, select the best and eliminate the inferior, and eliminate the abnormal growth in time. Zhongjing Global Purification can provide consulting, planning, design, construction, installation, transformation and other supporting services for edible fungi factories and edible fungi purification workshops.

Slope cryopreservation method is the simplest and most common strain preservation method, and it is also the most commonly used strain preservation method. It is suitable for almost all edible strains. First, the target strains should be inoculated into fresh slants. On the culture medium, cultivate at a suitable temperature, and after the mycelium grows over the entire slant of the test tube, put it in a 4 ℃ refrigerator for preservation; the slant cryopreservation method is suitable for short-term preservation of strains, and the preservation time is 3-6 months. When the deadline is approaching, the tube should be transferred in time, preferably once every 2-3 months. When transferring the tube, aseptic operation must be performed to prevent the contamination of miscellaneous bacteria. Aging, the preserved strains should be taken out of the refrigerator 1-2 days in advance when they are used, and they can be transferred to the tube after the vitality is restored after proper temperature cultivation. The filter paper preservation method is a method of adsorbing the spores of edible fungi on sterile filter paper and drying them for long-term preservation. Filter paper is selected to prepare filter paper strips, black filter paper is used for white spores, and white filter paper is used for other color spores; the filter paper is cut into 2 lengths. -3cm, 0.5cm wide filter paper strips, lay flat in a small test tube, and sterilize with high pressure steam at 0.15MPa for 30min; filter paper sheets store strains for a long time, the strains are not easy to age, the seed production is simple, and the storage and transportation are convenient.

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