Biological product production equipment in biological cell laboratory
Biological product production equipment in biological cell laboratory
In the modern product industry, biological products benefit from the development of biotechnology. In addition to the development of basic theories of biotechnology such as genetic recombination technology, the development of biotechnology is largely the development of biotechnology equipment, especially computer technology and nanomaterials...
Text label: cell laboratory, biological cell laboratory, biological product production equipment
Biological products production equipment in biological cell laboratory
In the modern product industry, biological products benefit from the development of biotechnology. In addition to the development of basic theories of biotechnology such as gene recombination technology, the development of biotechnology is largely the development of biotechnology equipment, especially computer technology and nanomaterial technology.
Nowadays biological products plants have many similarities with other GMP plants, such as the need for water and steam systems, clean gas, clean rooms, etc. However, the biological product process has extremely high specificity and complexity, resulting in the diversity and complexity of biological product equipment. At the same time, the detection of biological products is also different from other products, and a large number of biological or biochemical methods are used. Therefore, the testing instruments also have many particularities.
In terms of process flow, biological product equipment can be basically classified according to the process flow shown in the figure below:
Biological product process flow diagram
（1）Cell bank preparation and storage equipment
Cell Bank usually includes Primary Cell Bank (PCB), Master Cell Bank (MCB) and Work Cell Bank (WCB). According to industry regulations, each cell bank should be kept below -130°C (usually in a liquid nitrogen tank).
The preparation of cell bank is usually a process of expansion by original cells, and the scale of this stage is usually not too large. Therefore, equipment such as shakers and incubators are generally selected, and the main parameters of process control generally include: temperature, CO2 concentration, shaker speed, etc. Control equipment includes microscopes, inverted microscopes, biochemical analyzers, cell counters, etc.
（2）Cell recovery process equipment
The cell recovery process generally uses a water bath to thaw the frozen cells, inoculates them in a biological safety cabinet, and then transfers them to an incubator for culture. Shakers may be used in the process, and some require CO2 culture. The containers for cultivation include conical flasks, square flasks, Kjeldahl flasks, etc. In this process, chicken embryos are used as culture cells, and equipment such as incubators, inoculation machines, etc. are selected. Zhongjing Global Purification can provide consulting, planning, design, construction, installation and transformation of GMP workshop, purification workshop, sterile workshop, biological laboratory, cell laboratory and other supporting services.
(3) Cell culture and passage process
The trend of this process is to use bioreactors for culture, and now the development of microcarrier technology can make bioreactors suitable for adherent and non-adherent cell culture, expanding its use. The traditional adherent culture process usually uses a bottle spinner, or static culture.
(4) Harvest
In the harvesting process, the extracellular fluid is generally harvested, or the intracellular material is harvested after the cells are broken. Both methods will encounter the problem of host cell removal, so the clarification and filtration system is a common equipment, which will be used in the harvesting process. A large number of collection tanks, transfer tanks, etc.
(5) Purification and refinement
At present, the most used purification process is chromatography, and salting-out precipitation + chromatography are also commonly used. Therefore, chromatography system and various tube and tank systems are the main equipment for purification and purification. Ultrafiltration concentration and Ultrafiltration systems are often used in liquid exchange, various centrifuges are also commonly used equipment in the process of product separation, and sterilization filtration systems are also commonly used and necessary equipment.
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