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Disinfection and sterilization of inoculation environment and utensils in edible fungi workshop

In the production of bacteria in the edible fungus workshop, the disinfection and sterilization of the inoculation environment and utensils can be divided into physical methods and chemical methods; the disinfection and sterilization methods of the inoculation environment are related to the equipment. Negative ion generator; inoculation...

Text label: Edible mushroom factory decoration, Edible mushroom workshop design and decoration, Inoculation environment utensils sterilization

Disinfection and sterilization of inoculation environment and utensils in edible fungi workshop

In the production of bacteria in the edible fungus workshop, the disinfection and sterilization of the inoculation environment and utensils can be divided into physical methods and chemical methods; the disinfection and sterilization methods of the inoculation environment are related to the equipment. Workbench, negative ion generator; inoculation box and inoculation clean room modular require the installation of ultraviolet sterilization lamps and fluorescent lamps; ultra-clean workbenches use the principle of filtration sterilization, first filter the air to obtain sterile air, and then remove the sterile air from the wind. The hole is punched out to make the working range into a sterile state, and ultraviolet sterilization lamps and fluorescent lamps are also installed on the top of the workbench.

The negative ion generator is a way to sterilize the air by using strong ionization. By ionization, the bacterial protein and nucleic acid are denatured, and the sterilization effect is ideal. Ultraviolet light is part of sunlight, with a wavelength of 100-400nm, of which the ultraviolet light with a wavelength of 257nm is most effective for microorganisms. When microorganisms are irradiated by ultraviolet light, the DNA of cells absorbs energy. Use ultraviolet light for disinfection and sterilization, turn on the light 30 minutes before use, and set up a dark environment at the same time; the penetrating power of ultraviolet light is very weak, easy to be absorbed by solids, cannot penetrate thicker glass and paper, and is only suitable for surface Disinfection and disinfection of air and water; when the humidity in the air exceeds 55%, the bactericidal effect of ultraviolet rays decreases rapidly; it is necessary to prevent direct exposure of ultraviolet rays to the human body to avoid damage to the skin and conjunctiva; ultraviolet rays may induce harmful changes in the environment. Indirectly affect the growth of microorganisms, such as the production of ozone in the air, the production of hydrogen peroxide in the water, and the production of organic peroxides in the culture medium. Zhongjing Global Purification can provide consulting, planning, design, construction, installation, transformation and other supporting services for edible fungi purification workshops and edible fungi factories.

Inoculation utensils mainly include metal tools, glass utensils, high temperature plastic utensils, etc., including: basic utensils, such as test tubes, strain bottles, plastic bags, spore collectors, balances, petri dishes, funnels, gauze , Inoculating loops, hooks, shovels, tweezers, scissors, etc. The bacterial culture bottle is generally a glass bottle with a capacity of 750ML and a diameter of 3cm, and ordinary canned bottles and wide-mouthed bottles are also used. Filament growth and bacterial contamination. Commonly used sterilization methods are: dry heat sterilization, ultraviolet lamp irradiation disinfection and sterilization, and wastewater disinfection. Dry heat sterilization method includes flame burning method and baking sterilization method; baking sterilization is sterilization with hot air, the sterilization temperature is 140-170 ℃, and the sterilization time is 2-3H, which is suitable for metal and For the sterilization of glassware, the electric heating drying box is a common equipment for dry heat sterilization. The advantage is that it is convenient and thorough.

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