Design, construction and installation of a four-level biosafety laboratory

Design, construction and installation of a four-level biosafety laboratory

The four-level biological safety protection laboratory is divided into safety cabinet type laboratory and wearing positive pressure clothing type laboratory. In the safety cabinet type laboratory, all microbial operations are carried out in the Class III biological safety cabinet; In the positive pressure suit laboratory, the staff must wear special...

Text label: four-level biosafety laboratory, design, construction and installation, Zhongjing Global purification

Design, construction and installation of four-level biosafety laboratory

The four-level biological safety protection pharma machinery is divided into safety cabinet type laboratory and wearing positive pressure clothing type laboratory. In the safety cabinet type laboratory, all microbial operations are carried out in the Class III biological safety cabinet; In the positive pressure suit type laboratory, the staff must wear special protective clothing of the positive pressure suit type; the laboratory should be built in a separate building or a separate area within the building; It consists of a room and an entry passage, and the entry passage has at least three parts, which are the outer dressing room, the shower room and the inner dressing room in sequence; there should be an automatic interlocking device between adjacent doors to prevent two adjacent doors from being opened at the same time; Materials, items and equipment that cannot be brought into or out of the safety cabinet from the locker room should be installed on the wall of the safety cabinet with an autoclave with a double-door structure, and a immersion disinfection tank, a fumigation room or a ventilation transmission with a disinfection device. Window for transfer or disinfection; a buffer zone can be set up around the safety cabinet, which is a circular corridor or buffer room, which is part of the core area. exit channel.

The walls, floors, ceilings, etc. of the safety cabinet room and the inner dressing room should form a sealed inner shell, the floor should be sealed as a whole, the corners should be arc-shaped, the inner surface of the room should be water-discharged, corrosion-resistant, and all gaps in the structure should be should be sealed; all the sewer pipes on the floor of the safety cabinet lead directly to the liquid disinfection system, and the sewer openings and other service lines are installed with high-efficiency filters to prevent pests from entering; For the double-door autoclave for sterilizing the items transferred from the safety cabinet and the safety cabinet room, the outer door of the autoclave is opened outside the laboratory, and the gap must be well sealed; all equipment for transferring items inside and outside the laboratory must have a double-door structure. There must also be an automatic interlocking device between the two doors, and any windows must be shatter-proof and sealed. The four-level biosafety laboratory must be installed with a well-designed and constructed in-line ventilation system. The air intake and exhaust design of the system should ensure that the directional airflow flows from the least dangerous area to the greatest potential danger area. The four corners of the experimental area are reduced to a minimum; automatic monitoring and alarm devices for the ventilation system need to be designed and installed to ensure that there is no positive pressure in the laboratory and to maintain normal pressure and pressure difference in each room. The exhaust air of the Class III biological safety cabinet must be directly It is connected to the exhaust duct. The exhaust duct must be set up separately and must not be connected to the building exhaust system; the safety cabinet room must maintain the highest degree of negative pressure, and its relative pressure shall not be higher than -60Pa. The safety cabinet room, inner changing room, shower The relative pressure of the indoor and outdoor locker rooms increases in turn, and there should be a pressure difference between adjacent rooms, which should be kept at 10-15Pa; the artificial lighting in the laboratory should be uniform, not dazzling, and the illumination should not be lower than 500Ix; the air inlet should be a three-stage filter System, the last level must be filtered by high-efficiency filter; the air inlet and exhaust high-efficiency filters must be installed in the air vents on the maintenance structure in each room of the laboratory to avoid contamination of the air duct, and the high-efficiency filter air outlet structure must be replaced. Before the high-efficiency filter, it can be sterilized on the spot, or a filter structure that can be replaced in an air-tight bag is adopted, and the high-efficiency filter is sterilized later. One test; the exhaust air from the entire core area must be continuously processed by two high-efficiency filters, and the air outlet should be kept away from the laboratory area and the air inlet. Zhongjing Global Purification can provide consulting, planning, design, construction, installation, transformation and other supporting services for four-level biosafety laboratories and GMP laboratories.

The surface of the laboratory bench should be seamless or sealed, impervious to water, corrosion-resistant and heat-resistant; laboratory furniture should be simple, open-structured and strong, with space between the laboratory bench, safety cabinet and other equipment There is space for cleaning and disinfection. The corners and protruding parts of furniture and equipment should be smooth, free of burrs, and preferably arc-shaped; the laboratory and the outside must be equipped with a communication system, and a closed-circuit television system should be installed; in the safety cabinet room , A non-manually operated automatic hand basin is installed near the door of the inner and outer locker rooms. The door of the autoclave must be automatically controlled, and the outer door can only be opened after the sterilization cycle is completed; a double-door liquid soaking tank, a fumigation and disinfection room or a ventilation air lock room for disinfection must be provided. Classified biological safety cabinets and safety cabinets that cannot be autoclaved are sterilized so that they can enter and exit safely; if there is a central vacuum pipeline system, they should not be used outside the safety cabinet, and the online high-efficiency filter should be as close to each At the point of use or the cut-off door, the filter should be easy to be sterilized or replaced on site, and other gas and liquid pipelines leading to the safety cabinet should be equipped with protective devices to prevent backflow; it must be the core area of u200bu200bthe laboratory, the safety cabinet, the inner changing room, Ventilation systems, lighting, alarms, access controls and biological safety cabinets in showers and outside changing rooms are provided with automatic activation of emergency power.

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