Design and construction of animal biosafety laboratory
Design and construction of animal biosafety laboratory
Animal biosafety laboratories can be divided into four categories according to relevant standards: ABSL-1, ABSL-2, ABSL-3, and ABSL-4. ABSL-1 is suitable for laboratories housing most quarantined laboratory animals: the rearing room should be isolated from the rest of the building; the doors of the rearing room should have...
Text label: Design and construction of animal laboratory, biosafety laboratory, Zhongjing Global Purification
Design and construction of animal biosafety laboratory
Animal biosafety laboratories can be divided into four categories according to relevant standards: ABSL-1, ABSL-2, ABSL-3, and ABSL-4. ABSL-1 is suitable for laboratories that keep most quarantined experimental animals: the rearing room should be isolated from other areas in the building; the door of the rearing room should have a viewing window, open inward, and the opened door should be able to close automatically. The working surface should be waterproof and easy to sterilize and sterilize; windows should not be installed. If windows are installed, all windows should be sealed, and protective nets should be installed outside the windows when necessary; the strength of the enclosure structure should be consistent with the species of animals raised. Adaptation; if there is a ground liquid collection system, a liquid backflow device should be set up, and the water trap should have sufficient depth; the air exhausted from the animal pharma machinery should not be recycled; a sink or hand cleaning device should be installed, which should be installed at the exit; The indoor air pressure in the animal breeding room should be controlled to be negative pressure; experimental animal breeding cages or guardrails should be set up, and in addition to safety requirements, the requirements for animal welfare should also be considered; relevant waste disposal facilities and equipment should comply with relevant national regulations. .
ABSL-2 is suitable for microbial and animal experimental operations with moderate potential hazards to humans and the environment: when applicable, it should meet the requirements of ABSL-1; animal breeding rooms should be provided with buffer rooms at the entrance and exit; should be equipped in adjacent areas High-pressure steam sterilizer; the indoor air pressure in the animal breeding room should be controlled to a negative pressure, and the gas should be filtered through a HEPA filter and then directly discharged to other buildings; it should be used in a safety isolation device that may generate harmful aerosols The exhaust should be filtered by a HEPA filter and discharged; the external air outlet of the laboratory should be at least 2m above the top of the building where the laboratory is located, and it should be designed to prevent wind, rain, insects, and mice, but it should not affect The gas is discharged to the air; the sewage should be disinfected and sterilized, and the disinfection and sterilization effect should be monitored to ensure that the discharge requirements are met.
ABSL-3 is suitable for microorganisms and laboratory operations that are highly potentially dangerous to people and the environment, and should meet the requirements of ABSL-2 when applicable; there is a shower room in the laboratory protection area, and a compulsory shower device should be set up when necessary; The breeding room should be located in the middle of the entire laboratory as much as possible, and should not be directly adjacent to other public areas; if there is an entrance or exit in the breeding room, a buffer room should be set up; when the safety isolation device cannot be effectively used to raise animals, it should be Determine the biosafety protection requirements of the pharma machinery according to further risk assessment; monitoring equipment and communication equipment should be installed in the animal breeding room, portable local disinfection and sterilization devices should be equipped, and sufficient suitable disinfection and sterilization agents should be provided; there should be devices and technology for cleaning and reliable disinfection and sterilization of animal cages; devices and techniques should be available to clean and reliably disinfect and sterilize all items or the surfaces of their packaging before they are shipped out of the animal housing, where necessary; should be based on a risk assessment Appropriately handle the sewage in the shower room in the protection area, and monitor the sterilization effect to ensure that the discharge requirements are met; for laboratories that cannot effectively use the safety isolation device to operate the routine volume, the animal breeding room should be determined according to the results of the risk assessment. Whether the exhausted gas needs to be filtered by a two-stage HEPA filter and discharged. Zhongjing Global Purification can provide consulting, planning, design, construction, installation, transformation and other supporting services for animal laboratories and cell laboratories.
ABSL-4 is suitable for laboratory work of microorganisms and animals with high potential danger to humans and the environment. The shower room should be equipped with a compulsory shower device; the buffer room of the animal breeding room should be airlocked; there should be strict restrictions on entering the animal breeding room Access control measures for rooms; there should be devices and techniques for cleaning and reliable disinfection and sterilization of all items or the surfaces of their packaging before they are transported out of the animal room; the air tightness of the animal room and its buffer room should be sufficient to be tested when closed. All passages in the room and maintaining the temperature in the room at the upper limit of the design range, when the air pressure in the room rises to 500Pa, the air pressure that naturally decays within 20min is less than 250Pa; the pressure difference between the air pressure in the animal breeding room and the outdoor atmospheric pressure should not be If it is less than 100Pa, the negative pressure of the pressure difference with the adjacent area should not be less than 25Pa.
For those engaged in invertebrate operation laboratories, they should enter the animal breeding room through the buffer room. The buffer room should be equipped with adapted insect traps, and the door should be installed with gauze to prevent arthropods from escaping; Install gauze to prevent arthropods from escaping on the doors and windows that can be opened; it should have the conditions for airtightness and overall disinfection and sterilization; should be equipped with a spray-type insecticidal device; There are mechanisms to ensure that the liquid or sterilization solution in the sinks and traps does not dry up; monitor and record the number of arthropod larvae and adults that can fly, crawl, and jump; have the use of biological safety cabinets or equivalent safety isolation Apparatus to handle infected arthropods; monitoring and communication equipment should be provided as needed.
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