Airflow Design in PCR Laboratory - Cleanroom Related
First of all, the experimental areas must be independent of each other and cannot be directly connected. Each independent experimental area has a special buffer room for staff to change their work clothes and shoes. Entering each work area must strictly follow a single direction sequence, that is, only from the reagent preparation area on the left , sample preparation area, amplification reaction area 1, purification area, amplification reaction area 2 to post-purification area to avoid cross-contamination. In order to avoid cross-contamination between samples, the room adopts purification control, and the overall design is 100,000. The buffer room is designed as a positive pressure area to ensure isolation from the external environment and avoid aerosol pollution entering this area from adjacent areas. The flow of people and logistics is designed in different ways to avoid cross-mixing. A public corridor is designed in the whole area, and a special window for the transfer of reagents and items is designed between the working areas, so that people can be separated. Logistics: Electronic interlocking stainless steel transfer windows are designed between the reagent preparation area and the sample preparation area, between the sample preparation area and the PCR amplification area, and between the amplification area and the purification area. The prepared reagents are transferred from the reagent preparation area to the sample preparation area through the one-way transfer window, and the processed samples are transferred from the sample preparation area to the PCR amplification area through the one-way transfer window. The one-way item conveying system not only ensures that the sample reagents are not contaminated, but also ensures that the sample reagents do not pollute the environment. People flow: Personnel enter their respective experimental areas through the lock room, and when entering and leaving each experimental area, they need to change clean work clothes and hand disinfection in the lock room area.