Tissue culture workshop laboratory engineering design and decoration scheme
The layout of the tissue culture laboratory belongs to the category of architecture. Since the tissue culture room requires the separation of people and materials, the separation of clean and sewage, and to maintain the static pressure gradient of each clean operation room, the independent front room is centrally set in each clean room. The air lock, the air lock chamber is connected with each operation room at the same time,...
Text label: tissue culture laboratory design and decoration, tissue culture workshop engineering plan
Laboratory engineering design and decoration scheme of tissue culture workshop
The layout of the tissue culture laboratory belongs to the category of architecture. Since the tissue culture room requires the separation of people and materials, the separation of clean and sewage, and to maintain the static pressure gradient of each clean operation room, the independent front room is centrally set in each clean room. The air lock, the air lock room and each operation room are connected at the same time to ensure that the air in the low clean area does not penetrate into the high clean area; the staff in the sterile area change their shoes through the changing room - changing clothes - air shower room - inoculation room and cultivation room; The room logistics enters the buffer corridor from the outer corridor after being sterilized by the mechanical interlocking self-disinfection transfer window, and then enters each operating room through the stainless steel transfer window; the size of the tissue culture laboratory can be designed according to the nature of work and production scale, and the area can be Large or small, mainly used for scientific research and small-scale production, small area, large-scale factory production, large area, also often called tissue culture factory.
The tissue culture laboratory can be divided into different functional areas, mainly including washing and sterilization room, preparation room, culture clean room modular, aseptic operation room, and buffer room. The preparation room carries out all the preparations related to the experiment, and completes the storage, weighing, dissolving, utensil washing, medium preparation and distribution, sterilization of medium and culture vessels, and pretreatment of culture materials for various items used. It is best to have about 20m2, which is required to be spacious and bright, so as to facilitate the placement of multiple test benches and related equipment, so that many people can work at the same time; at the same time, good ventilation conditions are required to facilitate gas exchange; the laboratory floor should be easy to clean, and should be non-slip deal with. The washing and sterilizing room completes the washing, drying, preservation, sterilization of the culture medium, etc. of various utensils; the size of the washing and sterilizing room is determined according to the size of the workload. A dedicated washing tank is used to clean glassware; the central laboratory table should also be equipped with 2 sinks for cleaning small glassware; prepare 1-2 washing liquid tanks, which are specially used for washing glassware that requires high cleanliness , the ground should be moisture-resistant and well-drained. The inoculation room is mainly used for disinfection of plant materials, inoculation, transfer of culture, subculture of test-tube seedlings, preparation of protoplasts and all technical procedures that require aseptic operation. It is the most critical in vitro culture research or production of plants. One step; the inoculation room should be small but not large, generally 10-20m2, which requires good sealing, dry, quiet, clean and bright, and can be kept sterile for a long time. Smooth, easy to clean and sterilize; equipped with sliding doors to reduce air disturbance when opening and closing the door; for the convenience of sterilization, the floor and inner walls should be made of waterproof and corrosion-resistant materials; in order to keep clean, the sterile room should prevent air convection; The inoculation room requires 1-2 ultraviolet sterilization lamps to be hoisted in appropriate positions for irradiation sterilization. Zhongjing Global Purification can provide consulting, planning, design, construction, installation, transformation and other supporting services for tissue culture laboratories and tissue culture workshops.
Cultivation room is used to cultivate in vitro plant materials inoculated into culture flasks and other vessels under controlled conditions, whether it is a research laboratory or a place where productive experimental materials are cultivated; about 10-20m2, the space of the culture room The size can be determined according to the size, number, and other ancillary equipment of the culture rack, and its design is based on the principle of making full use of space and saving energy; the basic requirement is to be able to control light and temperature, and to maintain a relatively sterile environment; to meet the needs of plants For the needs of gas for the growth of culture materials, ventilation devices such as exhaust windows and ventilation fans should be installed in the cultivation room; in order to save energy and space, appropriate cultivation racks should be configured and fluorescent lamps should be installed. The buffer room is a buffer site before entering the sterile room to reduce the introduction of dust and other pollutants by the human body from the outside world. The staff put on work clothes, slippers and masks before entering the sterile room and culture room; entering the sterile room Change clothes and shoes here before the operation room to reduce the bacteria brought into the inoculation room when entering and leaving; the buffer room needs to be 3-5m2, which can be built in the preparation room or outside the sterile operation room, and should be kept clean and sterile; shoe racks and clothes are provided. cap hooks, clean experimental slippers and sterilized work clothes; 1-2 UV lamps are regularly irradiated on the top of the wall to sterilize the clothes; the door of the buffer room should be staggered from the door of the inoculation room, two Do not open the door at the same time to ensure that the sterile room does not bring in bacteria due to the door opening and the entry and exit of people.
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