Atmospheric dust is the object to be directly dealt with in the sterile room. In clean technology, the most commonly used counting concentration is based on the number of particles ≥ 0.5 μm. For the cleanest stratosphere (10km from the surface), such The particles are about 20 grains/L, and about 2600 grains/L above the very clean sea. The counted concentrations on land vary greatly, and the same area differs greatly from time to time. my country's atmospheric count concentrations can be roughly divided into 'industrial cities', 'urban suburbs', and 'non-industrial areas or rural areas'. The concentration of particles ≥ 0.5μm They are 1×106 grains/L, 2×105 grains/L and 3×105 grains/L, respectively. The concentration of atmospheric bacteria The pollution of atmospheric dust belongs to the pollution of inanimate particles, while the pollution of atmospheric bacteria belongs to the pollution of living particles. Microorganisms include: viruses, rickettsia, bacteria, fungi, protozoa. But it is mainly bacteria and fungi that are associated with cleanrooms. However, bacteria cannot survive alone, and generally attach to dust particles, so the meaningful size is its equivalent diameter. When a group of bacteria-carrying particles passes through the filter, the filtration efficiency of bacteria is the same as that of particles of a certain size, then this The particle size is the equivalent diameter of the group of bacteria-carrying particles. The amount of dust generated by internal pollution refers to the amount of dust generated in the sterile room. The amount of dust generated from the equipment can be considered to be eliminated through local exhaust, and not into the clean room; the amount of dust generated during the handling of products, materials, etc. small and can be ignored. Due to the current clean interior decoration using better metal siding, the amount of dust generated from the surface of the building is also very small, generally accounting for less than 10%, and the dust mainly comes from people, accounting for about 90%. p2 laboratory: 1. Comply with BSL-2 pharma machinery construction requirements. 2. The door of the pharma machinery is locked and can be automatically closed. doors should have viewing windows. 3. There is enough storage space to place items for easy use. There is also storage space for long-term use in the laboratory work area. 4. There should be conditions for storing personal clothing in the work area of the laboratory. 6. Equipped with high pressure steam sterilizers in all buildings of the laboratory, and inspected and verified regularly to ensure compliance with the requirements. 7. Equipped with biological safety cabinets in the laboratory. 8. Equipped with eyewash facilities and emergency sprinkler if necessary. 9. Consider mechanical ventilation, such as using windows for natural ventilation and insect screens. 10. Have reliable power supply and emergency lighting. When necessary, important equipment such as incubators, biological safety cabinets, refrigerators and other pharma machinery should be powered by power. 11. exits are clearly identifiable in the dark.
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