Mobile PCR square cabin laboratory supply and installation
Mobile PCR square cabin laboratory supply and installation
Design parameters of PCR square cabin laboratory: calculated temperature of indoor air conditioner in summer: 24±3℃, relative humidity: 30-70%; calculated temperature of indoor air conditioner in winter: 20±3℃. The 'specimen preparation area' is easy to be a negative pressure or enhanced P2 laboratory, and nucleic acid operations should be carried out in a biological safety cabinet...
Text label: mobile shelter, PCR laboratory supply and installation, mobile PCR shelter
Mobile PCR shelter laboratory supply and installation
Design parameters of PCR square cabin laboratory: calculated temperature of indoor air conditioner in summer: 24±3℃, relative humidity: 30-70%; calculated temperature of indoor air conditioner in winter: 20±3℃. The 'specimen preparation area' is likely to be a negative pressure or enhanced P2 laboratory, and nucleic acid operations should be carried out in a biological safety cabinet; the laboratory's three waste treatment, waste, waste water, and waste gas. Key point control: biological isolation, primary isolation, secondary barrier; absolute negative pressure to avoid indoor potential pollution efflux; streamline design, experimental items, experimental waste, experimental personnel in and out; airflow organization, indoor directional flow, Flow from the low pollution risk area to the high pollution risk area; the airflow organization adopts side-up and side-down discharge, and the arrangement of air supply and exhaust ports should minimize the space where the indoor airflow is stagnant; the high-efficiency filter exhaust port should be located in The area with the highest risk of indoor pollution should be arranged in one direction, without obstacles, and the exhaust duct should not be replaced by an exhaust sandwich wall; exhaust air treatment, centralized wastewater treatment, and discharge after sterilization.
The PCR laboratory is divided into 3 areas, which are reagent preparation, sample preparation, amplification and analysis areas; the buffers of the three PCR test areas are strictly separated and cannot be mixed to avoid inaccurate experimental data; experimental items only It can enter along the path of reagent preparation-sample preparation-amplification analysis, and cannot transfer items in reverse; in order to prevent the reverse airflow, the pressure difference in the three regions is controlled by means of differential pressure control. Therefore, the laboratory adopts fresh air and full exhaust ventilation. For laboratory testing, in order to avoid cross-contamination, double channels are set up. Personnel enter and leave separately in and out of different channels, and separate isolation clothing and isolation clothing pollution to avoid contamination of the dressing room by dirty isolation clothing; laboratory specimen entry and exit: through the dirt channel, through the The transfer window directly enters the specimen preparation; the laboratory adopts an access control system, only authorized experimental personnel can enter, the buffer room is interlocked, and the two doors cannot be opened at the same time to prevent the laboratory pressure difference from being inverted. Zhongjing Global Purification can provide design, supply and installation of PCR square cabin laboratory, and can provide consulting, planning, design, construction, installation, transformation and other supporting services of PCR laboratory.
Mobile PCR shelter laboratory design:
Partition settings: three areas: reagent storage and preparation area, sample preparation area, amplification product analysis
Buffer room setting: The buffer room is a necessary facility to block the air exchange in the room.
Air flow organization: flow from low-risk areas to high-risk areas.
Pressure gradient: Reagent storage and preparation area: clean clean room modular, should be protected from nucleic acid contamination, set to +10pa; sample preparation area: prevent nucleic acid spread and personnel infection, while preventing sample contamination, set to -10pa; gene Analysis of amplified region and amplified product: To prevent nucleic acid leakage, set to -20pa.
Hand disinfection: It is not easy to set water to wash hands, and alcohol disinfection should be set.
Indoor sterilization: UV lamps are used for irradiation sterilization, and indoor sterilization has dead spots. An ozone sterilizer is set in the air-conditioning unit, and the automatic control system is set to sterilization mode. The sterilization time is 45 minutes. The sterilization time is to manually turn on the mode after each test.
Biosafety Cabinet: A2 Biological Safety Cabinet.
Item transfer: transfer through the transfer window; material 304 stainless steel; specification 600*600*600; UV germicidal lamps are set inside.
Eyewash: set as eyewash bottle; double-bottle eyewash; wall-mounted arrangement.
Exhaust setting: block the virus out of the laboratory, set high-efficiency filters for exhaust air, and incinerate waste filters.
Dirt treatment: Dirt is divided into: waste, waste gas, waste water; waste treatment: packaging, professional incineration; waste gas: mainly laboratory exhaust air, which is treated by high-efficiency filters.
Mobile assembly: It can be hoisted and moved.
Test bench: all-steel test bench; with the ground and wall: glass glue, rivets fixed.
Clean benches, biological safety cabinets, refrigerators, and instruments can be fixed with brackets on the bench, or placed under the test bench, filled with inflatable shock absorbers;
Noise less than 65FB.
Weak current: set up an integrated network system, set up network points in each laboratory to facilitate data transmission; set up telephones to facilitate calls in various areas, and can also choose a conference system, three areas and several outdoor parties; set up a monitoring system to record the whole process laboratory process.
Strong electricity: power supply 220V, 50hz; because it is outdoor, in order to protect the instrument, the indoor total power supply should be protected against lightning strikes.
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